Abstrait

The role of miR-139-5p in non-small cell lung cancer (NSCLC) with inactivation of HCCS1 gene

Zhifeng Lin, Liwen Xiong, Qiang Lin, Ying Chen, Xiangnan Xu

Objective: This research was to study the inactivation of HCCS1 gene in Chinese patients with Non- Small Cell Lung Cancer (NSCLC) and the mechanism of MicroRNA 139-5p in the inactivation process.

Methods: 51 clinical cases with non-small cell lung cancer were collected and were detected by immunohistochemistry, to study the expression of HCCS1 (hVPS53) protein and MicroRNA 139-5p in 51 cases of NSCLC carcinoma tissue and carcinoma side normal tissue, and the relationship between HCCS1 (hVPS53) expression levels and clinical pathological analysis the characteristics of NSCLC, the relationship between the and the expression of MicroRNA 139-5p and HCCS1 (hVPS53).

Results: Immunohistochemistry analysis of HCCSI (hVpS53) and MicroRNA39-5p levels in 51 carcinoma tissues. We found that HCCSI (hVpS53) protein expression level in carcinoma tissue was 4.68 ± 1.15 (relative expression), and 6.14 ± 1.24 (relative expression) in the carcinima side normal tissue. The expression level of MicroRNA39-5p in carcinoma tissue was 4.21 ± 0.38, and in the carcinima side normal tissue, the expression level was 2.15 ± 0.22. Analysis of HCCS1 (hVPS53) and MicroRNA 139-5p protein, their expression had significant difference, however none difference correlated with gender, age and pathological type, TNM staging and tumor grade (P>0.05), however there was a significant difference in the TNM stage and tumor type.

Conclusion: As a tumor suppressor gene, HCCS1 (hVPS53) gene has abnormal changes in many tumor tissues. This study shows that the MicroRNA 139-5p methylation acted on Chinese HCCS1 non-small cell lung cancer gene suppression caused by the loss of heterozygosity and abnormal methylation of gene silencing, may be the mechanism of HCCS1 gene inactivation.