Abstrait

The effect of human adipose tissue derived stem cells-conditioned medium and Rosmarinus officinalis L.'s extract on the survival of breast cancer cells (MCF7) in vitro.

Motaghi Moridani Fatemeh, Haji Ghasem Kashani Maryam

Cultured Human Adipose Tissue Derived Stem Cells (hADSCs) secreted many growth factors, which called Conditioned Medium (CM). CM has antioxidant and antitumor activities. Recent studies have demonstrated antiproliferative and apoptotic features of Rosmarinus officinalis L.'s Extract (RE) on cancer cells.

In this study, CM was prepared from fourth passage of cultured hADSCs in serum-free medium for 48 h. MCF7 cells proliferation rate, survival and also expression of apoptotic genes treated by RE (50, 100, and 200 μg/ml) and CM for 24, 48, and 72 h were assessed by MTT, and RT-PCR.

Treated MCF7 cells by RE at concentration of 50 μg/ml at all times and CM at 24 and 48 h post induction have shown significant decrease of cell proliferation rate as compared to un-induced cells (control). Caspase 3 gene expression in cells treated by RE50 (24 and 72 h), RE100 or CM for 24 and 48 h was increased significantly as compared to control. In addition, significant increase of Caspase 9 gene expression was observed in 50 μg/ml RE- treated cells after 24, 48 and 72 h and 100 μg/ml RE- treated cells for 48 h as compared to control. While CM-induced cells showed a significant increase in the mRNA level of Caspase 9 gene at 24 h. Incubation with RE at 50 μg/ml for 24, 48 and 72 h or CM for 24 h were efficient for apoptosis induction of MCF7 cells. RE and CM treatment showed different effects on MCF7 cells in the absence or presence of serum, like a double-edged sword.