Abstrait

Association of MMP-1, 9, 12 and TIMP-1 gene polymorphisms in Malaysian male hypertensive subjects

Farizeh Aalam Ghomi Tabatabaee, Ramachandran Vasudevan, Farzad Heidari, Somayeh Khazaei, Ali Etemad, Patimah Ismail

Background: Matrix Metalloproteinases (MMPs) plays an important role in hypertensive vascular stiffness, remodeling and dysfunction. Several studies have been reported the imbalanced MMP: TIMP (tissue inhibitors of metalloproteinase-1) ratio in hypertensive subjects, indicating the depressed systematic degradation of collagenase in etiology of hypertension. The main objective of this study was to determine the candidate gene polymorphisms involved in extracellular matrix metabolism among Malaysian male subject with Essential Hypertension (EH).

Methods: A total of 133 newly diagnosed EH subjects and 129 unrelated healthy individuals were included in this study. The genomic DNA was extracted and the genotyping was done by PCR-RFLP method.

Result: The demographic characteristic of the subjects such as age, body mass index, systolic blood pressure, diastolic blood pressure, low-density lipoprotein, triglyceride and cholesterol were shown to be significantly different (p?0.05) in case subjects when compared to controls. The allelic distribution of TIMP-1 372 T/C gene polymorphism was significantly associated with hypertension (p?0.05). While, MMP-1: rs1799750, MMP-9: rs3918242 and rs17576 and MMP-12: rs2276109 polymorphisms did not differ significantly (p?0.05).

Conclusion: Hence, the rs4898 polymorphism of TIMP-1 may be considered as a possible genetic biomarker and a risk factor predictor for EH among Malaysian male subjects.