Abstrait

Analysis on constituents and drug resistance of pathogenic bacteria causing chronic obstructive pulmonary disease

Liu Nian, Xie Liangyi, Jiang Gang, Liu Wei, Jiang Yongliang

Objective: To separate the pathogenic bacteria of Chronic Obstructive Pulmonary Disease (COPD) and investigate their constituents and the resistance features of gram-negative bacilli, so as to provide some references for the clinical application of antibiotics in the respiratory department of Hunan Provincial People’s Hospital.

Methods: A total of 710 COPD patients who were admitted in the respiratory department of Hunan Provincial People’s Hospital from August 2013 to February 2017 were enrolled in the study. In order to get germ-free sputa, all of them had to rinse their mouth repeatedly with clean water or hydrogen peroxide. The sputa were assayed for drug sensitivity with the K-B methods drafted by National Committee for Clinical Laboratory Standards (NCCLS).

Results: A total of 813 strains of bacteria were isolated from 710 COPD patients, of which gramnegative bacilli were the major strains whose highlight was Pseudomonas aeruginosa. Gram-positive bacilli ranked second of which Staphylococcus aureus was the primary and fungi third. The strains and constituent ratio of gram-negative bacilli were significantly higher than that of gram-positive bacilli and that of fungi, and the difference was statistically significant (P<0.05). Through drug sensitive tests, it was known that Pseudomonas aeruginosa and Acinetobacter baumannii had higher resistance rate to cefazolin, with a statistical difference (P<0.05).

Conclusion: The highlight of bacteria-isolated is gram-negative bacteria which have very strong drug resistance, so that it is necessary to monitor unceasingly the resistance of bacteria, so as to guide reasonable medication in clinic.