Abstrait

Untargeted Metabolomics Based on Segmented Flow MS

Hussin Salih

Dimorphic yeast called Hyphozyma roseonigra is employed as a biocatalyst to change the plant triterpenoid sclareol into ambrosial. The latter is a step in the synthesis of ambrafuran, a highly valuable chemical used in the fragrance sector. Unfortunately, little is understood about the microorganism's fundamental biochemistry. In this study, the integration of multi-platform metabolomics was employed to gain a deeper biochemical understanding of H roseonigra. Using untargeted LC-MS and NMR techniques, the focus on metabolomics alterations during growth and development was achieved. Every second day, cells from the early, log, and stationary phases were extracted using extraction techniques that were platform-compatible from cell suspensions that had been cultivated in batch culture. The detected selective ions annotated from the endo and exometabolomes (metabo-fingerprinting and metabo-footprinting) were found to fall primarily in the primary metabolism class after chemometric analysis of LC-MS and NMR data gathered from both intra and extracellular extracts. Insights into the active metabolic pathways during growth and development were gained with the help of pathway mapping and feature-based network correlation analysis, which did not identify terpene production. This study sheds new light on the fundamental metabolic skills of H. roseonigra and suggests that the detoxification of a hydrophobic xenobiotic molecule occurs during the metabolism of sclareol.