Abstrait

Interaction caused the lipid content in jurkat t cells to increase.

Marta Carlo

Plasma membrane domains that form at T-cell antigen receptor (TCR) signalling foci transmit activating stimuli to T lymphocytes. In this study, the molecular lipid makeup of immunoisolated TCR activation domains was determined. In comparison to control plasma membrane fragments, we found that they accumulate cholesterol, sphingomyelin, and saturated phosphatidylcholine species. This presents the first direct evidence that the molecular lipid composition of TCR activation domains is distinct and resembles that of liquid-ordered raft phases in model membranes. Interestingly, plasmenyl phosphatidylethanolamine and phosphatidylserine were also enriched in TCR activation domains. Plasma membrane condensation at TCR signalling was compromised when polyunsaturated fatty acids were used to modify the T-cell lipidome. Foci and caused a disruption in the molecular lipid composition. These findings relate early TCR activation responses and specific plasma membrane condensation at sites of TCR activation to the accumulation of particular molecular lipid species.