Abstrait

Exploring the mechanism behind Alzheimers Disease in adult Zebra fish

Arti Singh

Keywords: Alzheimer’s disease; Memory dysfunction; Reserpine; Streptozotocin; Sopolamine; Zebrafish

Background: Zebrafish is emerging as a alternative vertebrate model for the study of memory dysfunction. Neuronal circuitries involved in learning and memory functions in Zebrafish are well characterized. With this model we can perform variety of behavior test, including learning and cognition, which has set a platform for use of this animal for behavioural pharmacology.

Aim: In this study we will establish the model of memory dysfunction by different methods like hypoxia-induced or chemical induced in adult Zebrafish and will try to uncover the mechanism behind memory dysfunction.

Objective: 1. To establish model of memory dysfunction in adult Zebrafish 2. To delineate the mechanism behind memory dysfunction in Zebrafish

Material and Methods: Adult Zebrafish (~3months old, maintained at 12 h light and 12 hr dark cycle) housed at our lab will be used for the study. Drugs like streptozotocin, reserpine, benzo-α-pyrene will be used to produce memory dysfunction in adult Zebrafish. Various parameters like behavioral (T-maze, Light and Dark chamber test), followed by biochemical and molecular analysis will be analysed.