Abstrait

Evaluation of the omentum as stem cell donor in the ischemic myocardium using immunohistochemical analysis of CD34

Luiz Fernando Kubrusly

 Objective: To evaluate the efficacy of omentopexy as stem cell donor, on previously infarcted myocardium, using immunohistochemically analysis with CD34. Methods: Myocardial infarction was generated in four pigs, by ligature of the 1st and 2nd marginal branches of the circumflex artery. In three animals, careful abrasion of the infarcted epicardium was performed followed by multiple myocardial perforations and the mobilization of the omentum from the abdominal cavity to the mediastinum, involving the infarcted area and perforations. In the fourth animal, omentopexy was not performed and only the abrasion and perforation of the infarcted area were performed. All hearts were removed for CD34 immunohistochemically evaluation. RESULTS: Four samples from different sites of each animal, totaling 16 histopathological samples were evaluated. All samples were immunolabelled for CD-34. After omentopexy, in 40% of the samples there was a 60% increase in CD34 immunohistochemical marking, and in the control animal, only minimal marking was observed. CONCLUSION: Omentopexy proved to be effective in continuously seeding previously infarcted myocardium with stem cells, seen through immunohistochemistry, using CD34 marker.