Abstrait

Effect of Xylaria nigripes, Panax notoginseng, Cuscuta chinensis on GABA, GABA transaminase, glutamate decarboxylase, amyloid beta, and amyloid beta aggregation levels in PC12 neuron cells.

Su-Fen Huang1, Han-Wen Yang1, Kamesh Venkatakrishnan2, Tsu-Chung Chang1*, Wen-Liang Chang3*

The purpose of this study is to determine the effect of three different botanical compositions, Xylaira Nigripes extract (XE), Xylaira Nigripes + Panax notoginseng extract (XPE), and Xylaira Nigripes+Panax notoginseng+Cuscuta chinensis extract (XPCE) on γ-Aminobutyric acid (GABA) content, GABA transaminase (GABA-T), Glutamate decarboxylase (GAD), cells viability, and Amyloid-β protein (Aβ) aggregation levels in PC12 neuron cell. For this study, different concentrations (40, 60, 80, and 120 μg/mL) of the three compositions were used and all the experiments were performed in triplicate. All three compositions could significantly improve (p<0.001) GABA production and also increase the activity of glutamate GAD along with decreased GABA-T activity in PC12 cells in a dose-dependent fashion. Moreover, all three compositions showed potent neuroprotective properties via increasing PC12 cell viability as well as significantly attenuating (p<0.001) the Aβ-induced injury and Aβ aggregation in a dose-dependent manner. Overall, all the three compositions exhibited strong neuroprotective activity by improving GABA release through elevated GAD activity with decreased GABA-T activity. Also, all three compositions enhance the proliferation (cell viability) of PC 12 cells and significantly lowers the Aβ aggregation. Out of the three compositions, XPCE (p<0.001) showed the best holistic neuroprotective and GABA releasing properties than XE and XPE.