Abstrait

Development and characterization of a gill cell line from striped catfish, Pangasianodon hypophthalmus, Sauvage (1878)

Mukunda Goswami*, A. Sathiyanarayanan, Nagpure N S, Gireesh Babu. P and Dhanjit Kumar Das

susceptibility, cytotoxicity, gene expression studies etc. The striped catfish, Pangasianodon hypophthalmus is one of the main species in Asian aquaculture, especially in countries like Thailand, Indonesia, China, India, Bangladesh and Vietnam. The present study reports the development of a permanent cell line from gill of P. hypophthalmus designated as PHG and its application in toxicological research. Leibovitz’s-15 (L-15) cell culture medium supplemented with 15% fetal bovine serum (FBS) was used for the maintenance of cell line PHG. The morphology of the PHG cell line was fibroblastic in nature. PHG cells grew well at varied temperatures ranging from 24 to 300C with an optimum temperature of 280C. The PHG cell line was characterized using sequence of mitochondrial cytochrome C oxidase subunit I, which authenticated the species of origin of the cell line. The cell line was transfected with pEGFP-C1 plasmid and transfection reporter gene was successfully expressed 48 hr post-transfection with 9% transfection efficiency. The toxicity assessment of two organophosphate pesticides, chlorpyrifos and malathion using PHG cell line revealed that the two organophosphate pesticides were cytotoxic to the cell line at varied concentrations.